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Research on rapid detecting microbial count in raw milk with ATP bioluminescence

Chunyan Li, Huo Gui-cheng, Deguo Wang, Wang Fu-hou

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Abstract

The research was aimed to detect the microbial count in raw milk with ATP bioluminescence,it was determined that the time of somatic cell lysis liquid was 3 and the optimal dilution multiple of milk sample was 20.ATP bioluminescence was adopted to detect microbial count of large batch of milk samples,at the same time,petri dish colony counting method of GB was used,the results indicated that log microbial count in raw milk had linear correlation with log fluorescence value,the correlation level was significant(P0.001).Regression equation of them was y=1.0496x+2.4.

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What this paper is about

The research was aimed to detect the microbial count in raw milk with ATP bioluminescence,it was determined that the time of somatic cell lysis liquid was 3 and the optimal dilution multiple of milk sample was 20.ATP bioluminescence was adopted to detect microbial count of large batch of milk samples,at the same time,petri dish colony counting method of GB was used,the results indicated that log microbial count in raw milk had linear correlation with log fluorescence value,the correlation level was significant(P0.001).Regression equation of them was y=1.0496x+2.4.

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Available abstract

The research was aimed to detect the microbial count in raw milk with ATP bioluminescence,it was determined that the time of somatic cell lysis liquid was 3 and the optimal dilution multiple of milk sample was 20.ATP bioluminescence was adopted to detect microbial count of large batch of milk samples,at the same time,petri dish colony counting method of GB was used,the results indicated that log microbial count in raw milk had linear correlation with log fluorescence value,the correlation level was significant(P0.001).Regression equation of them was y=1.0496x+2.4.

Key concepts: Bioluminescence, Lysis, Raw milk, Food science, Count data, Dilution, Petri dish, Plate count

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