Research on rapid assessment on aerobic bacterial count of raw milk using ATP bioluminescence

When assessing the aerobic bacterial count of raw milk using ATP bioluminescence,the results will be interfered by the abacterial ATP.A new pretreatment method was established in this research.Raw milk samples were pretreated before ATP determination,which was measured in relative light units(RLUs).And the Lumac ATP bioluminescence assay results were compared with standard plate counts(SPCs).The results indicated that ln-transformed SPCs in raw milk had linear correlation with ln-transformed RLUs(R2=0.982),and the correlation level was significant(P0.01).It was indicated that the pretreatment method established in this research could eliminate interference from the abacterial ATP efficiently,which would increase the accuracy of quantitative determination of the aerobic bacterial count in raw milk using ATP bioluminescence.