ScholarLens Back to search
2008•Journal of Yanan UniversityRequires access

Electro—chemiluminescence Immunoassay for Quantitatire determination of HCG

Jia Liang-yong

Open publisher page 0 citations

Abstract

To evaluate the reliability and clinical application of quantitative measuring serum HCG with ECLIA,according to the procedure described by NCCLS,the Serum HCG concentration in 62 clinical speci—ments was analyzed by ECLIA and RIA respectirely.the parameters such as precision,calibration curve stability,dilution recovery were determined.the results and the relativity between the two methods were compared.Although there were good repeativity and correlation between the two methods(r=0.964,P0.05).the recovery rates were all above 95%,the ECLIA appeared to have higher recovery rate(t=4.62,P0.05),the linear range of ECLIA was wider than that ofthe RIA.Conclusion s:The accuracy and precision of ECLIA were better than that of the RIA,ECLIA method is very usefm and suitable for clinical application.

About this research paper

What this paper is about

To evaluate the reliability and clinical application of quantitative measuring serum HCG with ECLIA,according to the procedure described by NCCLS,the Serum HCG concentration in 62 clinical speci—ments was analyzed by ECLIA and RIA respectirely.the parameters such as precision,calibration curve stability,dilution recovery were determined.the results and the relativity between the two methods were compared.Although there were good repeativity and correlation between the two methods(r=0.964,P0.05).the recovery rates were all above 95%,the ECLIA appeared to have higher recovery rate(t=4.62,P0.05),the linear range of ECLIA was wider than that ofthe RIA.Conclusion s:The accuracy and precision of ECLIA were better than that of the RIA,ECLIA method is very usefm and suitable for clinical application.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

To evaluate the reliability and clinical application of quantitative measuring serum HCG with ECLIA,according to the procedure described by NCCLS,the Serum HCG concentration in 62 clinical speci—ments was analyzed by ECLIA and RIA respectirely.the parameters such as precision,calibration curve stability,dilution recovery were determined.the results and the relativity between the two methods were compared.Although there were good repeativity and correlation between the two methods(r=0.964,P0.05).the recovery rates were all above 95%,the ECLIA appeared to have higher recovery rate(t=4.62,P0.05),the linear range of ECLIA was wider than that ofthe RIA.Conclusion s:The accuracy and precision of ECLIA were better than that of the RIA,ECLIA method is very usefm and suitable for clinical application.

Key concepts: Chemiluminescence, Reliability (semiconductor), Recovery rate, Immunoassay, Calibration curve, Chromatography, Dilution, Chemistry

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Electro—chemiluminescence Immunoassay for Quantitatire determination of HCG — Research Paper | ScholarLens