2009•Journal of Putian UniversityRequires access

Determination of Oleanolic Acid in the Sedum Aizoon by HPLC

Xueqin Xu

Abstract

Objective: To find out a method using high-performance liquid chromatography to measure Sedum aizoon oleanolic acid.Method: high-performance liquid chromatography with Hypersil ODS(5μm,250mm×4.0 mm) column,a mobile phase of methanol : 0.1 % phosphoric acid(80:20),detected the bones at 215 nm and tendons of grass in oleanolic acid content.The results showed that the linearity range of determination is 10.0—100.0μg·mL-1.The average recovery rate is100.4%.Conclusion: This method is simple,rapid,sensitive,accurate and reproducible.It can be used as a method determining the oleanolic acid in Sedum aizoon.

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Key concepts: Oleanolic acid, High-performance liquid chromatography, Phosphoric acid, Chromatography, Chemistry, Sedum, Biology, Botany