2007•Chinese Journal of Analysis LaboratoryRequires access

Determination of gallic acid in Sedum aizoon L.by HPLC

Yaoguo Wu

Abstract

A method for the determination of dissociated gallic acid and total gallic acid in Sedum aizoon L.was described.High preformance liquid chromatography was used.The determination was performed on a hypersil C18 column by UV detector at 273 nm with methanol-0.05% Phosphoric acid(15∶85) as the mobile phase.There was a linear correlation between the concentration of gallic acid(5.76～172.8 μg/mL) and the peak area value(r=0.9994).The average recovery was respectively 99.5% and 97.9% for dissociated gallic acid and total gallic acid.7 samples from different producing areas were determined.This method is convenient, accurate and can be used to identify and evaluate the quality of Sedum aizoon L..

About this research paper

What this paper is about

Why it matters

OpenAlex reports 1 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Gallic acid, Chemistry, High-performance liquid chromatography, Phosphoric acid, Chromatography, Biochemistry, Organic chemistry, Antioxidant