In Vitro Effects of Galangin on Cell Proliferation,Cycle Progression and Apoptosis of a Human Gastric Cancer SGC-7901 Cells

OBJECTIVE To investigate the effect of galangin on the anti-proliferation and apoptosis of gastric cancer SGC-7901 cells,and possible mechanisms.METHODS Proliferative activity of SGC-7901 cells was measured by MTT assay while that cell cycle progression and apoptosis of galangin-treated SGC-7901 cells were analyzed by flow cytometry,morphologic observation and mitochondrial membrane potential(MMP) analysis.RESULTSS The evaluated results showed that when galangin was added into cell culture in 40-200 μmol·L-1,proliferative inhibition occurred and was observed as a dose-and time-dependent manner.The calculated IC 50 values for galangin were 160,100 and 70 μmol·L-1when the treatment time was 24,48 and 72 h,respectively.Flow cytometry analysis revealed that the proportion of the cells in the G 2 / M phase was significantly enhanced from 4.40(control group) to 18.31(treatment group) by a treatment time of 24 h.CONCLUSION The cells in treatment group showed typical apoptotic morphology and a decrease in MMP.At the same time,the percentage of the apoptotic cells significantly increased from 2.6% or 4.3%(control group) to 27.4% or 65.6%(treatment group),when 160 μmol·L-1galangin treated SGC-7901 cells for 24 or 48 h.These mentioned results indicate that galangin can inhibit the proliferation and induce apoptosis of the SGC-7901 cells by perturbation in cell cycle progression and mitochondrial dysfunction.