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Determination of chlorogenic acid in Jinqiantonglin Oral Liquid by HPLC

Zhong Xiao-qun

Abstract

Objective: To control the quality of Jinqiantonglin Oral Liquid and establish the determination of chlorogenic acid in the liquid. Methods: HPLC was used to determine the content of chlorogenic acid on ODS-2 column with a mobile phase of methanol-acetonitrile-water(10.5∶2.5∶87). pH was adjusted to 3.0 by acetic acid. UV detection wavelength was set at 325nm. Results: The linearity of chlorogenic acid standard was well and the average recovery was 100.07%, RSD was 1.47%(n=6). Conclusion: This method is convenient, quick, accurate and available for the quality control of Jinqiantonglin Oral Liquid

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Key concepts: Chlorogenic acid, Chromatography, High-performance liquid chromatography, Chemistry, Acetic acid, Acetonitrile, Methanol, Organic chemistry