Expressions of P27 and cyclinD1 in the cancerous process from chronic atrophic gastritis and its significance

Objective To study the expressions and effects of P27 and cyclinD1 in the cancerous process from chronic atrophic gastritis accompanied by large-intestine-metaplasia to adenocarcinoma.Methods The expressions of P27 and cyclinD1 were determined in the specimens of normal gastric mucosa, chronic atrophic gastritis with large-intestine-metaplasia and gastric adenocarcinorma by immunohistochemistry(S-P).Results Positive immunostaining rate for P27 protein was the highest in normal gastric mucosa(75.0%,15/20) and decreased with progression from chronic atrophic gastritis to adenocarcinoma (40.0%,15.0% respectively).There were significant differences between chronic atrophic gastritis, gastric adenocarcinorma and normal gastric mucosa group(P0.05,P0.01 respectivel). Positive immuostaining of cyclinD1 from normal gastric mucosa (20.0%,4/20) to chronic atrophic gastritis (55.0%,11/20) to adenocarcinorma (75.0%,15/20) were increasing. There were dramatic differences between chronic atrophic gastritis, gastritis adenocarcinoma and normal gastric mucosa group(P0.05,P0.01). An interesting observation showed that inverse expression between P27 and cyclinD1 in most of the gastritis adenocarcinoma tested. The excessive expressions of cyclinD1 and inactivity of P27 were correlated with the invasive depth of adenocarcinoma and lymphaden metastasis.Conclusion These data have proved that P27 and cyclinD1 may be regarded as molecular marker of early carcinogenesis in chronic atrophic gastritis and be related to prognosis of gastric adenocarcinoma.