Water-extracting and Purifying Technology on Flavonoids from Hawthorn leaves

A technology for water-extracting and purifying flavonoids from hawthorn leaves with macroporous resin was studied.By means of water-extracting method,the optimum extraction conditions of flavonoids in hawthorn leaves were determined.The results showed that the optimum conditions of water-extracting were obtained as follows:the ratio of material to solvent is 1:20(g/mL),it is decocted 2 times and each time is sustained for 120 min.On the basis of these experiments,the average extraction rate of total flavonoids was 87.2 %.Coupling water-extraction to macroporous resin refinement,D101 type macroporous resin was used to separate and purify flavonoids from hawthorn leaves,the dynamic adsorption capacity of this resin was more than 80 % after repeating use 5 cycles.The elution ratio of flavonoids was above 86 % when the column of D101 type resin was eluted with 70 % ethanol.By using the above-mentioned technology,the recovery of product was 1.9 %,the purity reached 82.8 %.