Extraction and purification of flavonoids from chrysanthemum indicun

A method for extraction and purification of flavonoids from chrysanthemum indicun with macroporous resin was studied, with the content and recovery rate of flavonoids as indexes. The results are as flollows: The experiment of L9(34) by ethanol as extracting way, the optimum conditions of extracting flavonoids from chrysanthemum indicun are obtained. When the condition is 60% of density of ethanol, during 3 hours at 80 ℃ and extracting 3 times. The static adsorption capacity of AB-8 type resin was 114.65 mg/g, the static elution ratio were 94.9%, the dynamic adsorption capacity of AB-8 type resin was 94.5 mg/g, the recovery rate is more than 92.6% and the purity is more than 90%. AB-8 type resin is the best for separating and purificating chrysanthemum indicun in flavonoids. The optimum conditions is: the eluant is 70% alcohol and 2~3 times as the volume of the resin, the volume of the resin is 10.6 times of flavonoids in sample, concentration of flavonoids of sample is 19.8 mg/mL and current velocity of 2~3 mL/min, pH value of sample is 4~5.