2008•Xibei Linxueyuan xuebaoRequires access
Separation and Purification of Flavonoids from Walnut Leaves with Macroporous Resin
Xinhua Zhang
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Abstract
A method for separation and purification of flavonoids from walnut leaves with macroporous resin was studied,with the recovery rate of flavonoids as index.The results were as flollows: among six types of macroporous resin choosed,resin LS303 was the best one.The optimum process conditions were the loaded amount of flavonoids vs dry resin: 1∶8,the extract solution at a concentration about 4.67 mg·mL-1,pH 6.After eluted with 4BV of distilled water and 4BV of 70% ethanol,the yield of flavonoids was above 80% and product purity was above 70%.The process is simple,the flavonoids of walnut leaves can be separated and purified effectively.