Expression and significance of vascuIar endothelial growth factor and VIII-R Ag in human brain glioma

Objective To explore the relationship between the grade of glioma and the expression of VEGF and Ⅷ-R Ag by detection the expression of VEGF and Ⅷ-R Ag in human gliomas and normal brain tissues.Methods Three normal brain tissues were obtained from the other patients as the control group.Sixty-two tissue specimens were obtained from patients with human brain glioma.The tumors were diagnosed as low-grade astrocytoma in 19 cases,anaplastic astrocytoma in 20 cases,and glioblastoma in 23 cases according to the World Health Organization criteria(1990).Immunohistochemical staining of paraffin tissue sections of tumor and normal brain specimens were used for detection the expression of VEGF and Ⅷ-R Ag.The immunoreactive score of VEGF and Microvessel Density(MVD) were calculated.Results The expression of VEGF and MVD increased with the grade of glioma,and significant diference was found among the different grade of glioma;MVD increased with the grade of glioma,and significant diference was also found among all grades;The malignant grade of glioma was positively correlated to the expression of VEGF(r=0.941)and MVD(r=0.907).Conclusion This evidence suggests that VEGF play important role in forming and developing of human brain glioma by stimulation the Angiopoiesis.VEGF expression may reflect the proliferating activity of the tumor cell and prognosis in brain glioma.