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Determination of Resveratrol in the Roots of Polygonum Cuspidatum by HPLC

Hongjin Zhu

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Abstract

A simple and sensitive HPLC method for the determination of resveratrol in the roots of Polygonum cuspidatum was described,including the pretreatment of samples by polyamide column. The HPLC system consists of a reverse-phase C 18 Hypersil column (4.6 mm×200 mm) and a mobile phase of methanol-water(35∶65), with UV detective wavelength being 300 nm. The results showed that the calibration curve was linear in the range of 0.4～2.0 μg with r =0.9998 and the recovery was 98.19%.

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What this paper is about

A simple and sensitive HPLC method for the determination of resveratrol in the roots of Polygonum cuspidatum was described,including the pretreatment of samples by polyamide column. The HPLC system consists of a reverse-phase C 18 Hypersil column (4.6 mm×200 mm) and a mobile phase of methanol-water(35∶65), with UV detective wavelength being 300 nm. The results showed that the calibration curve was linear in the range of 0.4～2.0 μg with r =0.9998 and the recovery was 98.19%.

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Available abstract

A simple and sensitive HPLC method for the determination of resveratrol in the roots of Polygonum cuspidatum was described,including the pretreatment of samples by polyamide column. The HPLC system consists of a reverse-phase C 18 Hypersil column (4.6 mm×200 mm) and a mobile phase of methanol-water(35∶65), with UV detective wavelength being 300 nm. The results showed that the calibration curve was linear in the range of 0.4～2.0 μg with r =0.9998 and the recovery was 98.19%.

Key concepts: Chromatography, High-performance liquid chromatography, Resveratrol, Calibration curve, Chemistry, Polygonum, Methanol, Polygonaceae

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