Drug resistance monitoring of clinical isolates during 2009-2010

OBJECTIVE To understand the drug resistance of clinical isolates to common antibiotics in our hospital during 2009-2010. METHODS A medical bacteria detection system from Zhuhai DL Medical Biotech Co.LTD was used,and the MIC method was used for susceptibility testing,to retrospectively analyze clinical isolates in the past two years. RESULTS A total of 1586 pathogenic bacteria were isolated during 2009-2010.Among them,Gram-negative bacilli accounted for 64.9%,of which the detection rates of Escherichia coli,Klebsiella pneumoniae and ESBLs-producing Proteus mirabilis were 69.5%,48.5% and 4.5%,respectively.Gram-positive cocci accounted for 35.1%,in which the positive rates of methicillin-resistant Staphylococcus aureus(MRSA) and methicillin-resistant coagulase-negative staphylococci(MRCNS) were 58.5% and 46.6% respectively.The top five isolation rates were E.coli,Pseudomonas aeruginosa,K.pneumoniae,Staphylococcus aureus,and S.epidermidis.Gram-negative bacteria were most sensitive to imipenem.No vancomycin intermediate and resistant-Staphylococcus were found.Gram-negative bacilli and Gram-positive cocci had varying degrees of drug resistance. CONCLUSION The bacterial resistance monitoring shows that hospitals should enhance the monitoring of pathogenic bacteria distribution and drug resistance,and antibiotics should be rationally used to reduce bacterial multi-drug resistance and the development and transmission of extensively drug-resistant strains.