Simultaneously determination of four organic acids in common fruit by HPLC

The four organic acids(tartaric acid,malic acid,citric and succinic acid)in four fruit were determined by reversed-phase high performance liquid chromatography(RP-HPLC),A VP-ODS C18 column(150×4.6mm),an ultraviolet length at 215nm,and 0.02mol/L KH2PO4(pH= 3.0) as the mobile phase were employed,the flow velocity is 1mL/min and the column oven temperature is 30℃,pH and other conditions affected the separation of chromatography was investigated.The HPLC can separate the four organic acids of four fruit in one time.The average recoveries of the four organic acids were in the range of 96.1% to 112.9%,the liner correlation coefficient is above 0.999.This method got a accurate and highly effective result.