Determination of Organic Acids in Apple Fruits by HPLC

Objective: To establish a method for determining organic acids in apple fruits by high performance liquid chromatography(HPLC).Methods: Organic acids were extracted from apple fruits by ultrasonic-assisted water extraction.An IC PAKTMION Exclusion(Waters,WAT010290) column was used to separate organic acids.The mobile phase was 0.01 mol/L H2SO4 with a flow rate of 0.5 mL/min.The column temperature and UV detection wavelength were set as 50 ℃ and 210 nm,respectively.Results: Under chosen experimental conditions,six organic acids could be successfully separated.All the calibration curves for these organic acids displayed a good linear relationship with correlation coefficients above 0.9998 and precision RSDs of 0.10%-1.59%(n = 5) and repeatability RSDs of 1.79%-4.26%(n = 5).The recovery rates of six organic acids were in the range of 91.05%-105.18%.Conclusion: The method is convenient,rapid,accurate and applicable to determine organic acids in apple fruits.