A Study on Simultaneous Determination of 7 kinds of Organic Acids and Vc in Dragon Fruit by RP- HPLC

The main factors that affect the separation of organic acids were studied,and a method for simultaneous and rapid determination of purple red flesh dragon fruit in oxalic acid,tartaric acid,malic acid,acetic acid,lactic acid,citric acid,succinic acid and Vc content with reverse-phase high performance liquid chromatography( RP-HPLC) was established. The chromatographic conditions are as follows: the column temperature was 30 ℃,the flow rate was 1 m L / min,the wavelength was 215 nm,the buffer was 0. 05 mol / L H3PO4-Na2HPO4 and a Kromasil column( 250 mm × 4. 6 mm) was adopted. The results showed that malic acid was the most important organic acid,the next was citric acid. Because this method can complete a determination within10 min. So it has the advantages of fast,high sensitivity and good repeatability,it is a high reference to the determination and research of metabolism of organic acid and Vc in fruit.