The study of DNA damage induced by arsenic, fluoride,CIT and T-2 toxin in cultured SGC-7901 cells by SCGE

Objective To study the features of DNA damage induced by arsenic, fluoride, citreoviridin (CIT) and T-2 toxin in cultured SG C-7901 cells.Methods Single cell gel electrophoresis(SCGE) and Komet Image Analysis System (KIAS) were used to measure the DNA damage of the cells t reated for 4 hours with 10 μmol/L sodium arsenite, sodium fluoride, CIT or T- 2 toxin,respectively.Results The tail lengths of damaged DNA were significan tly greater in the sodium arsenite group than those in the control group; the ta iled cell percentage in the sodium fluoride group was significantly higher than that in the control group; CIT significantly increased the tailed cell percentag e, tail length and tail moment; whereas in the T-2 toxin group, the tailed cell percentage, tail DNA, tail length and tail moment were all increased. Conclusions Sodium arsenite, sodium fluoride, CIT and T -2 toxin are all DNA damage inducers, but the DNA damages induced by them show different features, so their mechanisms of inducing DNA damage may be different.