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2005•Study Journal of Traditional Chinese MedicineRequires access

Determination of the Puerarin in the Ganmao Soft Capsule by HPLC

Yue Chen

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Abstract

Objective: To establish the quantitative method of the Puerarin in the Ganmao Soft capsule by HPLC. Method :The Diamonsil C18 Column (4.6mm× 150mm, 5μm) was used. The methanol-water (V: V = 25:75) mixture as the mobile phase, with the detection at 250nm, the flow rate was 1.0mL/min and column temperature was room temperature. Result:.The liner ranges are 0.1594-1.195μg (r = 0.99997, n = 5). The mean recovery is 102.0 % , RSD = 1.82 % (n = 5) . Conclusion:This method is convenient, accurate and specified.

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What this paper is about

Objective: To establish the quantitative method of the Puerarin in the Ganmao Soft capsule by HPLC. Method :The Diamonsil C18 Column (4.6mm× 150mm, 5μm) was used. The methanol-water (V: V = 25:75) mixture as the mobile phase, with the detection at 250nm, the flow rate was 1.0mL/min and column temperature was room temperature. Result:.The liner ranges are 0.1594-1.195μg (r = 0.99997, n = 5). The mean recovery is 102.0 % , RSD = 1.82 % (n = 5) . Conclusion:This method is convenient, accurate and specified.

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Available abstract

Objective: To establish the quantitative method of the Puerarin in the Ganmao Soft capsule by HPLC. Method :The Diamonsil C18 Column (4.6mm× 150mm, 5μm) was used. The methanol-water (V: V = 25:75) mixture as the mobile phase, with the detection at 250nm, the flow rate was 1.0mL/min and column temperature was room temperature. Result:.The liner ranges are 0.1594-1.195μg (r = 0.99997, n = 5). The mean recovery is 102.0 % , RSD = 1.82 % (n = 5) . Conclusion:This method is convenient, accurate and specified.

Key concepts: Puerarin, Capsule, Chromatography, Chemistry, High-performance liquid chromatography, Medicine, Botany, Biology

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