Simultaneous determination of three effective components in Houttuynia cordata thunb

To establish a method for the determination of chlorogenic acid,rutin and quercetin in different kinds of and different parts of houttuynia cordata thunb.The three compounds were distilled and their contents were determined by HPLC with 90% EtOH as solvent using soxtherm distill machine with diamonsilTMC18(4.6 mm×150 mm i.d.,5 μm) as a chromatographic column and 0.06 mol/L V(NaH2PO4)∶V(MeOH)=7.3∶2.7、 V(MeOH)∶V(H2O)∶V(HAc)=48∶50∶2 as the mobile phase.The flow rate is 1 mL/min,and the detection wavelength of chlorogenic acid,rutin and quercetin is 326 nm,254 nm,254 nm. The linear ranges of chlorogenic acid,rutin and quercetin are 4.6～30.7 μg/mL,0.30～7.5 μg/mL and 0.034～0.85 μg/mL and the average recoveries are 99.3%,98.9%,98.7% and RSDs are 1.0%,1.6%,0.7%,respectively.The method can be used for the quality control of Houttuynia cordata thunb materials and preparations.