Study on the Analysis Methods of Quercetin Content in Houttuynia cordata Thunb

[Objective]The aim was to establish a HPLC method for the content determination of natural quercetin in different parts of Houttuynia cordata Thunb.[Methods] The combination between the cellulase and ultrasonic extracting was used;and the chromatographic column was XDB-C18(4.6 mm×150 mm,5 μm),mobile phase was acetonitrile-1% acetic acid(40∶60),column temperature was 25 ℃,and the flow velocity was 0.8 ml/min,the detection wavelength was 370 nm.[Result] There was good linear relationship for linear within the range of 0.1-14.4 μg/ml(r=0.999 9) and the average recovery of quercetin in the roots of Houttuynia cordata Thunb samples was 99.2% and RSD was 3.9%.The average recovery of quercetin in the stems and leaves of Houttuynia cordata Thunb was 98.8% and RSD was 3.8%.[Conclusion]The established methods had many advantages,such as effective separating effect,speciality,simple operation,accurate results and good reproducibility,therefore,it can be applied for the determination of quercetin in Houttuynia cordata Thunb.