Determination of chlorogenic acid and quercetin in Houttuynia cordata Thunb. from different districts

Objective:To establish a high-preformance capillary electrophoresis(HPCE)method for determination of chlorogenic acid and quercetin in Houttuynia cordata Thunb.from different districts.Methods:The capillary pil- lar used in this study was capillary without smear layer,the buffer solution was pH 8.5 and 30 mmol·L~(-1)sodium borate solution,the running voltage was 16 kV,the wavelength of detection was 254 nm,and the column temperature was 25℃.Results:The detected concentration of chlorogenic acid and quercetin showed good linear relationships with the peak area in the ranges of 0.05-0.8 mg·mL~(-1)(r=0.9995)and 0.04-0.15 mg·mL~(-1)respectively. The average recoveries were 98.7% and 98.8%,with RSD of 2.2% and 2.5%(n=3).Conclusion:The method is simple,rapid,and good reproducibilities.It can be used to control the quality of Houttuynia cordata Thunb.