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2010•Zhongguo zhongyiyao xinxi zazhiRequires access

Determination of Chlorogenic Acid in Compound Oral Liquid of Kangbingdu by HPLC

Tang Dezhi

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Abstract

Objective To establish a method for detecting chlorogenic acid in compound oral liquid of Kangbingdu with HPLC.Methods The chromatographic column of Shim-pack VP-ODS C18(4.6 mm× 250 mm,5 μm) was used,the mobile phase was consisted of acetonitrile-0.2% phosphoric acid aqueous solution(12∶88).The flow rate was 1.0 mL/min,the detective wavelength was 327 nm and column temperature was 30 ℃.Results The linear range of the method was 0.051～0.922 μg(r=0.999 9,n=6),the average recovery was 99.04%,RSD was 1.31%(n=6).Conclusion The method is accurate,reproducible,simple and easy to do,which can be used in quality control of compound oral liquid of Kangbingdu.

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What this paper is about

Objective To establish a method for detecting chlorogenic acid in compound oral liquid of Kangbingdu with HPLC.Methods The chromatographic column of Shim-pack VP-ODS C18(4.6 mm× 250 mm,5 μm) was used,the mobile phase was consisted of acetonitrile-0.2% phosphoric acid aqueous solution(12∶88).The flow rate was 1.0 mL/min,the detective wavelength was 327 nm and column temperature was 30 ℃.Results The linear range of the method was 0.051～0.922 μg(r=0.999 9,n=6),the average recovery was 99.04%,RSD was 1.31%(n=6).Conclusion The method is accurate,reproducible,simple and easy to do,which can be used in quality control of compound oral liquid of Kangbingdu.

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Available abstract

Objective To establish a method for detecting chlorogenic acid in compound oral liquid of Kangbingdu with HPLC.Methods The chromatographic column of Shim-pack VP-ODS C18(4.6 mm× 250 mm,5 μm) was used,the mobile phase was consisted of acetonitrile-0.2% phosphoric acid aqueous solution(12∶88).The flow rate was 1.0 mL/min,the detective wavelength was 327 nm and column temperature was 30 ℃.Results The linear range of the method was 0.051～0.922 μg(r=0.999 9,n=6),the average recovery was 99.04%,RSD was 1.31%(n=6).Conclusion The method is accurate,reproducible,simple and easy to do,which can be used in quality control of compound oral liquid of Kangbingdu.

Key concepts: Chromatography, Phosphoric acid, Chlorogenic acid, High-performance liquid chromatography, Chemistry, Acetonitrile, Linear range, Aqueous solution

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