Determination of mogroside V and 11-oxomogroside V in dried mature fruits of Momordica grosvenori by HPLC

Objective To establish an optimal HPLC method for determining the contents of mogro-side V and 11-oxomogroside V in the dried mature fruits of Momordicae grosvenori from various habitats and breeds. Methods ODS Column (250 mm× 4.6 mm, 5 μm) was used with acetonitrile-water as mobile phase in a gradient program, flow rate was 0.75 mL/min, detection wavelength was set at 210 nm, column temperature was 40℃. Results The liner range of mogroside V was 0.804 6-20.115 0 μg (r=0.999 8 ) and the average recovery of mogroside V was 104.6%, RSD was 3.28% (n=6). The liner range of 11-oxomogroside V was 0.598 5—14.962 5 μg (r=0.998 4) and the average recovery was 102.5%, RSD was 4.43% (n=6). Conclusion HPLC method is simple, rapid and accurate. It can be used for the quality control of the dried mature fruits of M. grosvenori.