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Determination of the Content of Vitamin C in Siraitia grosvenorii Fruits by HPLC

Fang Hong

Open publisher page 4 citations

Abstract

Objective:To set up a HPLC method for the determination of the content of vitamin C in the fruits of Siraitia grosvenorii. Methods:The chromatographic separation was achieved on SinoChrom ODS-BP (250 mm×4.6 mm,5μm) held at 25 ℃ using a mixture composed of 0.05% H3PO4 and CH3OH (98:2,V/V) as the mobile phase at a flow rate of 1.0 mL/min. The diode array detector wavelength was set at 242 nm. The injection volume used was 10μL. Results:The developed calibration curve of vitamin C displayed good linearity over the concentration range from 0.08 to 5.0 mg/mL,with a correlation coefficient equaling 1. The average recovery of five replicates for vitamin C was 99.3% with 1.93% relative standard deviation (RSD). Conclusion:This method is simple,quick,precise and stable,and can used for determining the content of vitamin C in the fruits of Siraitia grosvenorii.

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Objective:To set up a HPLC method for the determination of the content of vitamin C in the fruits of Siraitia grosvenorii. Methods:The chromatographic separation was achieved on SinoChrom ODS-BP (250 mm×4.6 mm,5μm) held at 25 ℃ using a mixture composed of 0.05% H3PO4 and CH3OH (98:2,V/V) as the mobile phase at a flow rate of 1.0 mL/min. The diode array detector wavelength was set at 242 nm. The injection volume used was 10μL. Results:The developed calibration curve of vitamin C displayed good linearity over the concentration range from 0.08 to 5.0 mg/mL,with a correlation coefficient equaling 1. The average recovery of five replicates for vitamin C was 99.3% with 1.93% relative standard deviation (RSD). Conclusion:This method is simple,quick,precise and stable,and can used for determining the content of vitamin C in the fruits of Siraitia grosvenorii.

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Available abstract

Objective:To set up a HPLC method for the determination of the content of vitamin C in the fruits of Siraitia grosvenorii. Methods:The chromatographic separation was achieved on SinoChrom ODS-BP (250 mm×4.6 mm,5μm) held at 25 ℃ using a mixture composed of 0.05% H3PO4 and CH3OH (98:2,V/V) as the mobile phase at a flow rate of 1.0 mL/min. The diode array detector wavelength was set at 242 nm. The injection volume used was 10μL. Results:The developed calibration curve of vitamin C displayed good linearity over the concentration range from 0.08 to 5.0 mg/mL,with a correlation coefficient equaling 1. The average recovery of five replicates for vitamin C was 99.3% with 1.93% relative standard deviation (RSD). Conclusion:This method is simple,quick,precise and stable,and can used for determining the content of vitamin C in the fruits of Siraitia grosvenorii.

Key concepts: Chromatography, High-performance liquid chromatography, Chemistry, Relative standard deviation, Vitamin C, Calibration curve, Vitamin, Chromatography detector

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