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Determination of mogroside V in liquor by HPLC

Xue Jiang

Open publisher page 1 citations

Abstract

To establish an optimal HPLC method for determining the contents of mogroside V in liquor,and gain the ends of detection of Momordica grosvenori infusion in liquor.Welch Materials Column-XB-C18(4.6×250mm,5μm)was used with acetonitrile-water(77∶23)as mobile phase,while flow rate was 1mL/min.Detection wavelength was set at 203nm according to the fixed UV detector.Column temperature was 25℃.The liner range of mogroside V was 0.4~20μg(R2=0.9983)while the range recovery of mogroside V was 97.5%~105.5% with 2.56% of RSD.HPLC method was simple,rapid,accurate,while with good precision,repeatability and stability.It can be used in the detection of momordica grosvenori infusion in liquor.

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What this paper is about

To establish an optimal HPLC method for determining the contents of mogroside V in liquor,and gain the ends of detection of Momordica grosvenori infusion in liquor.Welch Materials Column-XB-C18(4.6×250mm,5μm)was used with acetonitrile-water(77∶23)as mobile phase,while flow rate was 1mL/min.Detection wavelength was set at 203nm according to the fixed UV detector.Column temperature was 25℃.The liner range of mogroside V was 0.4~20μg(R2=0.9983)while the range recovery of mogroside V was 97.5%~105.5% with 2.56% of RSD.HPLC method was simple,rapid,accurate,while with good precision,repeatability and stability.It can be used in the detection of momordica grosvenori infusion in liquor.

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Available abstract

To establish an optimal HPLC method for determining the contents of mogroside V in liquor,and gain the ends of detection of Momordica grosvenori infusion in liquor.Welch Materials Column-XB-C18(4.6×250mm,5μm)was used with acetonitrile-water(77∶23)as mobile phase,while flow rate was 1mL/min.Detection wavelength was set at 203nm according to the fixed UV detector.Column temperature was 25℃.The liner range of mogroside V was 0.4~20μg(R2=0.9983)while the range recovery of mogroside V was 97.5%~105.5% with 2.56% of RSD.HPLC method was simple,rapid,accurate,while with good precision,repeatability and stability.It can be used in the detection of momordica grosvenori infusion in liquor.

Key concepts: Chromatography, Repeatability, Chemistry, High-performance liquid chromatography, Linear range, Recovery rate, Detection limit

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