Preparation of Mogroside V from the Fruits of Siraitia grosvenorii by Semi-Preparative HPLC

Objective:Semi-preparative HPLC was employed to establish a fast,stable and efficient method for preparation of high purity mogroside V.Methods:The crude mogroside V extract from the fruits of Siraitia grosvenorii was decolorized by D101 macroporous resin column chromatography before semi-preparative HPLC separation.Based on the use of Eclipse XDBC18(9.4mm× 250 mm,5μm) held at 38 ℃,the chromatographic conditions employed were as follows:mobile phase,a mixture of acetonitrile and water at a flow rate of 4 mL/min in the gradient elution mode,detection wavelength,210 nm;abd sample load 36 mg.Results:The purity of the product obtained was as high as 99.1%.Conclusion:The developed method,thanks to the benefits of simplicity,rapidity,good stability and obtaining of high purity products,will have a good application prospect.