2006•Huadong Shifan Daxue xuebao. Ziran kexue banRequires access
Regulation and Tissue Specific Expression of VHA-c3 of Arabidopsis Thaliana(Chinese)
Ping Xu, Xiaofang Li, Weijun Zeng, Shuiping Wang
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Abstract
Gene expression of VHA-c was detected in Arabidopsis silique,flower,leaf,bolt,root.However,the expression level of VHA-c3 was much higher in leaves than in the other tissues.Analysis of the putative promoter of VHA-c3 was carried out in tobacoo leave system.A series of the promoter mutants were constructed in which various extents of VHA-c3 promoter were placed upstream of the GUS gene in vector pC1305.1.The expression of GUS was analyzed by Agrobacterium-mediated transient expression system.It was found that a 2 812 bp promoter region has high gene expression capacity.And there is a high potential key cis-element for leaf-specific expression between 2 812～2 234 bp region.