Localization of Arabidopsis VHA-c3 Promoter with GUS-Fusion

VHA-c3 gene is one of the five homologous genes which belong to the subunits of vacuole H+-ATPase in Arabidopsis thaliana.Former reports indicated that it is related with resistance to abiotic stress in plants.Different fragments of the upstream regulation sequences of Arabidopsis VHA-c3 gene were cloned,and fused with GUS reporter gene,to study the localization of VHA-c3 gene.The essential element for the basal activity of VHA-c3 is located within the-722bp fragments upstream of the translation start codon of VHA-c3,it can guide genes' constitutive expression in leaf blade,trichome,petiole,root,stamen,stigma and sepal in Arabidopsis;And there is two negative regulatory elements located in-2812bp to-2234bp and-1496bp to-772bp respectively,and an up-regulating cis-element from-2234bp to-1496bp fragment of VHA-c3 gene,which may control the gene's expression in guard cell.