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2013•Journal of Henan Normal UniversityRequires access

Temperature and Light Regulates the Promoter Activity of VHA-c Genes of Arabidopsis Thaliana

Ruigang Wang

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Abstract

To analyze the promoter activity of VHA-c genes of Arabidopsis thaliana,The GUS repoter gene was fused with the promoter of VHA-c2,c3and c5respectively,and the VHA-c-GUSconstructs were transformed into tobacco leaves.Expression analysis of GUS activity under 4℃,40℃ or 25℃showed that all the three promoters were induced by low temperature,while the VHA-c3promoter was up-regulated under 40 ℃,but the VHA-c2promoter was inhibited slightly under the same conditions.Light also up-regulated GUS expression driven by the three promoters respectively,indicating that all the three promoters were responsive to light.

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What this paper is about

To analyze the promoter activity of VHA-c genes of Arabidopsis thaliana,The GUS repoter gene was fused with the promoter of VHA-c2,c3and c5respectively,and the VHA-c-GUSconstructs were transformed into tobacco leaves.Expression analysis of GUS activity under 4℃,40℃ or 25℃showed that all the three promoters were induced by low temperature,while the VHA-c3promoter was up-regulated under 40 ℃,but the VHA-c2promoter was inhibited slightly under the same conditions.Light also up-regulated GUS expression driven by the three promoters respectively,indicating that all the three promoters were responsive to light.

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Available abstract

To analyze the promoter activity of VHA-c genes of Arabidopsis thaliana,The GUS repoter gene was fused with the promoter of VHA-c2,c3and c5respectively,and the VHA-c-GUSconstructs were transformed into tobacco leaves.Expression analysis of GUS activity under 4℃,40℃ or 25℃showed that all the three promoters were induced by low temperature,while the VHA-c3promoter was up-regulated under 40 ℃,but the VHA-c2promoter was inhibited slightly under the same conditions.Light also up-regulated GUS expression driven by the three promoters respectively,indicating that all the three promoters were responsive to light.

Key concepts: Promoter, Arabidopsis thaliana, Promoter activity, Gene, Arabidopsis, Gene expression, Biology, Molecular biology

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