HPLC Fingerprinting of Extract of Erigeron Breviscapus

OBJECTIVE HPLC fingerprinting of the extract of Erigeron breviscapus was established.METHODS A Diamonsil C_ 18 column was used with mobile phase of 0.1% phosphoric acid and methanol with gradient elute.The flow rate was 0.6ml·min -1 ,at detection wavelength of 335nm.RESULTS The RSDs of relative retention time were less than 1% and the RSDs of relative peak area which are inspected were less than 3% in the precision examination .CONCLUSION The methods is simple ,accurate with good reproducibility.It may be practical value for the quality control of sample for Erigeron breviscapus.