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2006•The Chinese Journal of Modern Applied PharmacyRequires access

Methodology study on quantitative assay of Erigeron breviscapus

Zhang Yi

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Abstract

OBJECTIVE To establish a proper quantitative method for Erigeron breviscapus on the basis of the existed flaw methods.METHODS (1) High performance liquid chromatography and diotron array detection(HPLC-DAD): Column: Hypersil ODS2(250 4.6mm,5μm),mobile phase: acetonitrile-H_(2)O-H_(3)PO_(4)(18∶82∶0.2),detection wavelength was at 286nm,temperature was at 40℃;(2) UV spectrophotometry analysis. RESULTS(1) Caffeoyl conjugates and flavonoids were regarded as the staple in E.breviscapus.(2) The methods to determine scutellarin by HPLC and total phenols by UV were raised.CONCLUSION Quantifying scutellarin and total phenols altogether can effectively control the quality of E.breviscapus and its preparations.

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OBJECTIVE To establish a proper quantitative method for Erigeron breviscapus on the basis of the existed flaw methods.METHODS (1) High performance liquid chromatography and diotron array detection(HPLC-DAD): Column: Hypersil ODS2(250 4.6mm,5μm),mobile phase: acetonitrile-H_(2)O-H_(3)PO_(4)(18∶82∶0.2),detection wavelength was at 286nm,temperature was at 40℃;(2) UV spectrophotometry analysis. RESULTS(1) Caffeoyl conjugates and flavonoids were regarded as the staple in E.breviscapus.(2) The methods to determine scutellarin by HPLC and total phenols by UV were raised.CONCLUSION Quantifying scutellarin and total phenols altogether can effectively control the quality of E.breviscapus and its preparations.

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Available abstract

OBJECTIVE To establish a proper quantitative method for Erigeron breviscapus on the basis of the existed flaw methods.METHODS (1) High performance liquid chromatography and diotron array detection(HPLC-DAD): Column: Hypersil ODS2(250 4.6mm,5μm),mobile phase: acetonitrile-H_(2)O-H_(3)PO_(4)(18∶82∶0.2),detection wavelength was at 286nm,temperature was at 40℃;(2) UV spectrophotometry analysis. RESULTS(1) Caffeoyl conjugates and flavonoids were regarded as the staple in E.breviscapus.(2) The methods to determine scutellarin by HPLC and total phenols by UV were raised.CONCLUSION Quantifying scutellarin and total phenols altogether can effectively control the quality of E.breviscapus and its preparations.

Key concepts: Scutellarin, Chromatography, Chemistry, High-performance liquid chromatography, Phenols, Biochemistry

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