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RP-HPLC Determination of Secutellarin in Erigeron breviscapus(Vant.)Hand.-Mazz.

BI Kai-shun

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Abstract

Objective:To determine secutellarin in Erigeron breviscapus with RP-HPLC.Method:ODS 2 column (200mm×4.6mm,5μm)was used.The mobil phase consisted of aeetontrile-water-THF-H_3PO_4(15:85:1.5 :0.1),flow of rate:0.8mL·min~(-1) and temperature of column 30℃ and detected at 335 nm.Result:The linear range was 2.16-34.5μg·mL~(-1)for secutellarin.The method recoveries were 96.9%-97.5%,and the relative standard deviations were 1.5%-2.4%.Conclusion:This method is simple and can be used to determine secutel- latin with satisfactory accuracy and reproducibility.

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What this paper is about

Objective:To determine secutellarin in Erigeron breviscapus with RP-HPLC.Method:ODS 2 column (200mm×4.6mm,5μm)was used.The mobil phase consisted of aeetontrile-water-THF-H_3PO_4(15:85:1.5 :0.1),flow of rate:0.8mL·min~(-1) and temperature of column 30℃ and detected at 335 nm.Result:The linear range was 2.16-34.5μg·mL~(-1)for secutellarin.The method recoveries were 96.9%-97.5%,and the relative standard deviations were 1.5%-2.4%.Conclusion:This method is simple and can be used to determine secutel- latin with satisfactory accuracy and reproducibility.

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Available abstract

Objective:To determine secutellarin in Erigeron breviscapus with RP-HPLC.Method:ODS 2 column (200mm×4.6mm,5μm)was used.The mobil phase consisted of aeetontrile-water-THF-H_3PO_4(15:85:1.5 :0.1),flow of rate:0.8mL·min~(-1) and temperature of column 30℃ and detected at 335 nm.Result:The linear range was 2.16-34.5μg·mL~(-1)for secutellarin.The method recoveries were 96.9%-97.5%,and the relative standard deviations were 1.5%-2.4%.Conclusion:This method is simple and can be used to determine secutel- latin with satisfactory accuracy and reproducibility.

Key concepts: Chemistry, Chromatography, Reproducibility, High-performance liquid chromatography, Relative standard deviation, Linear range, Detection limit

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RP-HPLC Determination of Secutellarin in Erigeron breviscapus(Vant.)Hand.-Mazz. — Research Paper | ScholarLens