Study on 1 strain of Pseudomonas aeruginosa with multi-drug resistance gene

Objective To study the mechanism of multi-drug resistance of Pseudomonas aeruginosa.Methods The genes of multi-drug resistance including beta-lactamase,aminoglycosides modifying enzymes(AMEs),disinfect resistance and sulfanilamide-resistance were determined by polymerase chain reaction(PCR).The sequence of VIM gene was detected.Results The results of PCR indicated that aac(6')-Ⅰb,blaCARB,gyrA,oprD2,ant(2″)-Ⅰ,qacE1-sul1,blaIMP-I,blaTEM,blaVEB,aac(3)-Ⅱ,ant(3″)-Ⅰ,intⅠ1 and blaVIM were positive,while aac(3)-Ⅰ,aac(6')-Ⅱ,blaGES,blaGIM,blaOXA-10 group,blaPER,blaSPM,blaSHV and blaDHA genes were negative.The sequence of VIM PCR products of beta-lactamase genes was analyzed by BLAST and the result showed that it was VIM-2.Conclusion There are multi-drug resistance genes in the strain of Pseudomonas aeruginosa.The anti-bacterial effect of the second generation tube,of which the surface is imbrued with diamidines biguanidines and sulfanilamide need to be reevaluated.