Study on resistance mechanism and resistant genes of pan resistant Pseudomonas aeruginosa

Objective To investigate resistance-associated genes of pan resistant Pseudomonas aeruginosa.Methods We extracted DNA of pan resistant Pseudomonas aeruginosa isolated from clinical patients and detected β-lactamase genes,aminoglycoside modification enzymes(AMEs) genes and resistant antiseptic(qacE△ 1-sul1)gene with polymerase chain reaction(PCR).Results PCR results showed that the positive ratios of aac(6′)-Ⅰ b,blaCARB,gyrA,oprD2,ant(2″)-Ⅰ and qacE△ 1-sul1 genes were all 100%;blaIMP,blaTEM,blaVEB,aac(3′)-Ⅱ,ant(3″)-Ⅰ,intⅠ 1 and blaVIM genes were 95.5%,77.3%,13.6%,4.5%,4.5%,4.5% and 4.5%,respectively.While aac(3′)-Ⅰ、 aac(6′)-Ⅱ 、 blaGES、 blaGIM、 blaOXA-10、 blaPER、 blaSPM、 blaSHV and blaDHA genes were all negative.Conclusions The positive oprD2 genes indicated that outer-membrane proteins still existed in these Pseudomonas aeruginosa.The pan resistance of these Pseudomonas aeruginosa accounted for available multi-kinds of resistance associated genes.