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Relationship between ras p53 gene RNA and protein expression and HCC metastasis

Zheng Shi

Open publisher page 8 citations

Abstract

IM To understand the relationship between the expression of both ras and p53 gene RNA and protein and the metastasis of primary hepatocellular carcinoma (HCC).METHODS Immunohistochemistry and slot blot were used to study ras and p53 gene protein and RNA in 14 cases of HCC with metastasis and 16 cases of HCC without metastasis respectively.RESULTS After microwave treatment, the positive rates of both p21 and p53 protein in 14 cases of HCC with metastasis were significantly higher than that without metastasis (11/14 vs 5/16, P=0014 and 12/14 vs 7/16, P=0026, respectively). The results of slot blot showed that OD value of Nras RNA in HCC with metastasis was 3 to 5 times higher than that in HCC without metastasis, whereas OD value of p53 RNA had no difference between the two groups.CONCLUSION Immunohistochemistry of p21 and p53 protein after antigen retrieval is a useful index for patients with HCC metastatic potential

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IM To understand the relationship between the expression of both ras and p53 gene RNA and protein and the metastasis of primary hepatocellular carcinoma (HCC).METHODS Immunohistochemistry and slot blot were used to study ras and p53 gene protein and RNA in 14 cases of HCC with metastasis and 16 cases of HCC without metastasis respectively.RESULTS After microwave treatment, the positive rates of both p21 and p53 protein in 14 cases of HCC with metastasis were significantly higher than that without metastasis (11/14 vs 5/16, P=0014 and 12/14 vs 7/16, P=0026, respectively). The results of slot blot showed that OD value of Nras RNA in HCC with metastasis was 3 to 5 times higher than that in HCC without metastasis, whereas OD value of p53 RNA had no difference between the two groups.CONCLUSION Immunohistochemistry of p21 and p53 protein after antigen retrieval is a useful index for patients with HCC metastatic potential

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Available abstract

IM To understand the relationship between the expression of both ras and p53 gene RNA and protein and the metastasis of primary hepatocellular carcinoma (HCC).METHODS Immunohistochemistry and slot blot were used to study ras and p53 gene protein and RNA in 14 cases of HCC with metastasis and 16 cases of HCC without metastasis respectively.RESULTS After microwave treatment, the positive rates of both p21 and p53 protein in 14 cases of HCC with metastasis were significantly higher than that without metastasis (11/14 vs 5/16, P=0014 and 12/14 vs 7/16, P=0026, respectively). The results of slot blot showed that OD value of Nras RNA in HCC with metastasis was 3 to 5 times higher than that in HCC without metastasis, whereas OD value of p53 RNA had no difference between the two groups.CONCLUSION Immunohistochemistry of p21 and p53 protein after antigen retrieval is a useful index for patients with HCC metastatic potential

Key concepts: Immunohistochemistry, Metastasis, Hepatocellular carcinoma, Western blot, RNA, Cancer research, Gene, Messenger RNA

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