Determination of Germacrone in Superfine Powder of Different Processed Products of Curcuma kwangsiensis by HPLC

Objective: To determine the content of germacrone from different processed products of Curcuma kwangsiensis before and after the superfine comminution.Method: With the ball milling technology at low temperatures(-30 ± 2) ℃,the size of the superfine powder was between 1.0-2.0 μm.The HPLC analysis was performed on Hypersil ODS2 C18(4.6 mm × 250 mm,5 μm),the mobile phase was acetonitrile-water(65∶ 35),the detection wavelength was at 210 nm.Result: The germacrone content of commom powder(75-53 μm) was higher than the content of the superfine powder(1.0-2.0 μm).Conclusion: The size of superfine powder influenced the content of germacrone of the four different processed products of C.kwangsiensis evidently,and this offered referenced data for the different processed products of C.kwangsiensis.