Determination of the Germacrone in Folium Rhododendri Daurici by HPLC

Objective To develop a determination method of germacrone in the Folium Rhododendri daurici and determined samples which brought from different area in province of Heilingjiang and from different month of one area.Methods HPLC method was used with column of the Zorbax RX-C18柱(150 mm×4.6 mm,5μm)and the mobile phase of 65%acetonitrile.The velocity of flow was 1.0 ml/min and detection wavelength at 210nm.Results The linear range of germacrone is from 0.030 24μg to 3.024μg.(r=1.000).The recovery was 98.6%～101.5%with RSD of 1.3%～0.6%.The germacrone contents of the different area varied from 0.010 7 to 7.286 2 mg/g,while the different month reached 1.5 times.Conclusion The establishedmethod is accurate and reproducible,can be used for the determination of germacrone in Folium Rhododendri daurici.The content of germacrone was obvious difference for the 13 area's samples and was relatively near for the samples of 6 months.