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Establishment of regeneration system and callus inducing of Festuca ovina seeds

Wenjing Liu

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Abstract

The mature seed of the Festuca ovina taken as the explants to study the effects of different hormone compositions on callus induction,regeneration and rooting.In modified MS medium(MSM) with different concentrations of 2,4-D,KT,6-BA and NAA to establish fescue clones and efficient regeneration system.The results showed that the medium MSM+6.0 mg/L 2,4-D+0.02 mg/L KT was better for callus induction,the regeneration rate in medium of MSM+2.0 mg/L 6-BA+0.6 mg/L NAA was the highest,about 82%.The rooting rate reached 100% in 1/2MSM +0.6 mg/L NAA.The best regeneration made a sound foundation for the subsequent transgenic research.

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What this paper is about

The mature seed of the Festuca ovina taken as the explants to study the effects of different hormone compositions on callus induction,regeneration and rooting.In modified MS medium(MSM) with different concentrations of 2,4-D,KT,6-BA and NAA to establish fescue clones and efficient regeneration system.The results showed that the medium MSM+6.0 mg/L 2,4-D+0.02 mg/L KT was better for callus induction,the regeneration rate in medium of MSM+2.0 mg/L 6-BA+0.6 mg/L NAA was the highest,about 82%.The rooting rate reached 100% in 1/2MSM +0.6 mg/L NAA.The best regeneration made a sound foundation for the subsequent transgenic research.

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Available abstract

The mature seed of the Festuca ovina taken as the explants to study the effects of different hormone compositions on callus induction,regeneration and rooting.In modified MS medium(MSM) with different concentrations of 2,4-D,KT,6-BA and NAA to establish fescue clones and efficient regeneration system.The results showed that the medium MSM+6.0 mg/L 2,4-D+0.02 mg/L KT was better for callus induction,the regeneration rate in medium of MSM+2.0 mg/L 6-BA+0.6 mg/L NAA was the highest,about 82%.The rooting rate reached 100% in 1/2MSM +0.6 mg/L NAA.The best regeneration made a sound foundation for the subsequent transgenic research.

Key concepts: Callus, Regeneration (biology), Biology, Explant culture, Botany, Festuca, Horticulture, Poaceae

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