Experimental study of the myocardial protection of puerarin on the model of myocardial ischemia-reperfusion injury in Rabbits

Objective To investigate the effect of myocardial protection of puerarin on myocardial ischemia reperfusion injury in rabbits and its mechanism.Methods (1)Infarcted sizes in myocardial were estimated by dye method and the myocardial ultrastructure at the ischemia region was observed under the electron miscroscope.(2)The serum concentrations of superoxide dismutase(SOD)、plasma edothelin (ET) and the expression of bcl-2 protein in rabbits in groups pretreated by puerarin (50mg/kg,iv) were measured by radioimmunoassay and immunohistochemistry.Results Compared with the 0.9% sodium chloride groups, the infarcted size were significantly decreased (P0.01) and the damage of the myocardial ultrastrueture was significantly ameliorated in the puerarin-treated group;Puerarin can increase the serum concentrations of SOD and decrease the plasma concentrations of ET significantly (P0.05);Compared with the control group and 0.9% sodium chloride group,puerarin can significantly increased the expression of bcl-2 protein in ischemia reperfusion myocardial (P0.001).Conclusion Puerarin can reduce myocardial ischemia reperfusion injury significantly.The myocardial protection of puerarin may be realized by increasing the serum concentrations of SOD、reducing the plasma concentrations of ET and increasing the expression of bcl-2 protein.