Protection of PEGylated puerarin on myocardial ischemia-reperfusion injury in rats

Objective To investigate the protective effect of PEGylated puerarin on the myocardial ischemia-reperfusion injury(MIRI) in rats.Methods Sixty healthy SD rats were randomly divided into six groups: Sham,MIRI model,puerarin injection(20 mg/kg,positive control),low-,mid-,and high-dose PEGylated puerarin(244,488,and 976 mg/kg) groups.MIRI rat model was carried out by ligation of left anterior descending coronary artery for 30 min followed by reperfusion for 120 min.Different doses of PEGylated puerarin and puerarin injection were given 5 min after the ligation.Concomitant arrhythmias was surveyed by electrocardiogram,and the blood was collected at the end of reperfusion for detecting the changes of creatine kinase(CK),lactic dehydrogenase(LDH),aspartate aminotransferase(AST),and malondialdehyde(MDA).The myocardial infarct size was also estimated.Results Both puerarin injection and PEGylated puerarin exhibited the antiarrhythmic activity,decreased the contents of CK,LDH,AST,and MDA,and reduced the myocardial infarct size.The efficacy of mid-and high-dose PEGylated puerarin groups was better than that of puerarin injection group.Conclusion PEGylated puerarin exerts the protective effects on MIRI in rats.