2004•Chinese journal of integrated traditional and Western medicineRequires access
Protective effects of puerarin on myocardial ischemia/reperfusion injury in rabbits
Bingquan Shen
Open publisher page 1 citations
Abstract
Objective: To investigate the mechanism of puerarin against myocardial ischemia/reperfusion injury. Methods: The myocardial ischemia/reperfusion injury model of rabbits were reproduced by ligating the left ventricular anterior coronary artery, and by the fourchannel physiology recorder, the hemodynamic and the function of left ventricular were recorded. Forty rabbits were randomly divided into three groups: 10 animals were in the normal group , 10 animals were in the shamoperated control group ( n =10), and the other 20 animals reproduced myocardial ischemia/reperfusion injury models, which were randomly divided into model and puerarin subgroups. The puerarin group was injected puerarin injection intravenously at 20 minutes before ligating and at the beginning of reperfusion. Lipid peroxide (LPO), superoxide dismutase(SOD) and glutathione peroxidase(GSHPx) were detected at 40 minutes after ischemia and at 20 minutes after reperfusion . The ischemia/reperfusion injuried myocardial tissue of 0 5 g was sampled and LPO, SOD, GSHPx as well as GSHPx/LPO were detected in myocardial homogenate. Results: In the puerarin group, the left ventricular summit pressure, maximum rate of intraventricular pressure rise of left ventricular, maximum rate of intraventricular pressure down of left ventricular, and GSHPx/LPO in serum were all increased significantly( P 0 05 or P 0 01), serum LPO decreased significantly( P 0 05), LPO in myocardial tissue decreased, SOD and GSHPx/LPO in myocardial tissue increased significantly (all P 0 05) compared to the model group. Conclusion: Puerarin has the protective effect on ischemia/reperfusion injured myocardium by scavenging oxygen free radicals and antilipid peroxidation reaction.