Quality Comparison of Evodianmine and Rutaecarpine in Wuji Pills Using Different Production Process

Objective:To establish a HPLC method for the content of evodianmine and rutaecarpine in Wuji pills by HPLC,and to compare them between ultramicro Wuji pills and general Wuji pills.Methods:Chromatographic column:Hypersil C18(4.6 mm×250 mm,5 μm),mobile phase:acetonitrile-water(48:52),flow rate:1 mL/min,wavelength 225 nm.Results:The number of theoretical plates calculated by evodiamine and rutaecarpine peak was not below 3000.The regression equation of ultramicro evodiamine was A = 107.73 ρ-14.331,r = 0.999 9 and the linear range was 1.02-20.40 μg/mL.The regression equation of Ultramicro rutaecarpine was A = 15.596 ρ-45.09,r = 0.999 8 and the linear range was 4.4-176 μg/mL.The average recovery of ultramicro evodiamine was 99.61%(n = 5),RSD 0.50%,and the mass fraction of ultramicro evodiamine was(0.096±0.0148)%.The average recovery of Ultramicro rutaecarpine was 94.85%(n = 5),RSD 1.0%,and the mass fraction of ultramicro rutaecarpine was(0.343±0.0467)%.The average recovery of general evodiamine was 98.56%(n = 5),RSD 1.70%,and the mass fraction of general evodiamine was(0.81±0.003)%.The average recovery of General rutaecarpine was 99.55%(n = 5),RSD 0.50%,and the mass fraction of general rutaecarpine was(0.161±0.007 5)%.Conclusion:The mass fraction of evodiamine and rutaecarpine in ultramicro Wuji pills is higher than that of general Wuji pills.