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Determination of Chlorogenic Acid in Flos lonicerae by HPLC

Shuzhen Luo

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Abstract

The objectives to appraised Flos lonicerae's quality by the content of chlorogenic acid.Chlorogenic acid were extracted with Soxhlet extraction method,the content of chlorogenic acid in Flos lonicerae was determined by HPLC.The results indicated that detecting wavelength was 242 nm,the method was linear at chlorogenic acid ranging from 0.20 to 1.00 μg,RSD was 1.08,typical equation was Y=30 517.234 X+4.433,r=0.999 9(n=5),the content of chlorogenic acid was 2.6%.Therefore,Flos lonicera with good quality had great value on development and utilization.

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What this paper is about

The objectives to appraised Flos lonicerae's quality by the content of chlorogenic acid.Chlorogenic acid were extracted with Soxhlet extraction method,the content of chlorogenic acid in Flos lonicerae was determined by HPLC.The results indicated that detecting wavelength was 242 nm,the method was linear at chlorogenic acid ranging from 0.20 to 1.00 μg,RSD was 1.08,typical equation was Y=30 517.234 X+4.433,r=0.999 9(n=5),the content of chlorogenic acid was 2.6%.Therefore,Flos lonicera with good quality had great value on development and utilization.

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Available abstract

The objectives to appraised Flos lonicerae's quality by the content of chlorogenic acid.Chlorogenic acid were extracted with Soxhlet extraction method,the content of chlorogenic acid in Flos lonicerae was determined by HPLC.The results indicated that detecting wavelength was 242 nm,the method was linear at chlorogenic acid ranging from 0.20 to 1.00 μg,RSD was 1.08,typical equation was Y=30 517.234 X+4.433,r=0.999 9(n=5),the content of chlorogenic acid was 2.6%.Therefore,Flos lonicera with good quality had great value on development and utilization.

Key concepts: Chlorogenic acid, Flos, Chromatography, Chemistry, High-performance liquid chromatography, Extraction (chemistry), Food science, Biochemistry

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