ScholarLens Back to search
2006•Journal of Shandong Institute of Light IndustryRequires access

Determination of chlorogenic acid in the extraction of Flos Lonicerae by reversed-phase HPLC

Ying-Qin Wei

Open publisher page 0 citations

Abstract

To make a rapid and accurate method for determination of chlorogenic acid in the extraction of Flos Lonicerae by reversed-phase HPLC.Experiments were carried out on a Kromasil C18 collumn(250mm×(4.6)mm,5μm);mobile phase:a solution of 0.3% phosphoric acid-acetonitrile(90∶10,V/V);detection wavelength:327nm;injection volume:20μL.Chlorogenic acid in the extraction of Flos Lonicerae was well separated.Chlorogenic acid had a good linearity in the range of 0.0256～0.0896 mg·mL and the average recovery rate was 97.58%.The new method was suitable for determination of chlorogenic acid in the extraction of Flos Lonicerae by reversed-phase HPLC.

About this research paper

What this paper is about

To make a rapid and accurate method for determination of chlorogenic acid in the extraction of Flos Lonicerae by reversed-phase HPLC.Experiments were carried out on a Kromasil C18 collumn(250mm×(4.6)mm,5μm);mobile phase:a solution of 0.3% phosphoric acid-acetonitrile(90∶10,V/V);detection wavelength:327nm;injection volume:20μL.Chlorogenic acid in the extraction of Flos Lonicerae was well separated.Chlorogenic acid had a good linearity in the range of 0.0256～0.0896 mg·mL and the average recovery rate was 97.58%.The new method was suitable for determination of chlorogenic acid in the extraction of Flos Lonicerae by reversed-phase HPLC.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

To make a rapid and accurate method for determination of chlorogenic acid in the extraction of Flos Lonicerae by reversed-phase HPLC.Experiments were carried out on a Kromasil C18 collumn(250mm×(4.6)mm,5μm);mobile phase:a solution of 0.3% phosphoric acid-acetonitrile(90∶10,V/V);detection wavelength:327nm;injection volume:20μL.Chlorogenic acid in the extraction of Flos Lonicerae was well separated.Chlorogenic acid had a good linearity in the range of 0.0256～0.0896 mg·mL and the average recovery rate was 97.58%.The new method was suitable for determination of chlorogenic acid in the extraction of Flos Lonicerae by reversed-phase HPLC.

Key concepts: Chlorogenic acid, Flos, Chromatography, High-performance liquid chromatography, Chemistry, Phosphoric acid, Extraction (chemistry), Acetonitrile

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of chlorogenic acid in the extraction of Flos Lonicerae by reversed-phase HPLC — Research Paper | ScholarLens