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2014•Zhongguo zhongyiyao xinxi zazhiRequires access

Discussion on Content Determination Method of α-asarone in Dianxianqing Granules

Zhang Jing-ha

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Abstract

Objective To discuss the content determination method of α-asarone in Dianxianqing Granules. Methods Kromasil C18 column(250 mm×4.6 mm, 5 μm) was used, with methanol and water as mobile phase of gradient elution. The flow rate was 1.0 mL/min, and the detection wavelength was 257 nm. The column temperature was 25 ℃. Results The sample solution was prepared by reflux extracted with 70% ethanol for 20 min. α-asarone had a good linearity in the ranges of 1.24×10-3-7.44×10-3 μg(r =0.999 5). The method was quite good in precision, stability and repeatability, and the average recovery of α-asarone was 98.14%, with corresponding RSD of 2.5%. Conclusion The experiment provides an accurate and fast analytical method for α-asarone determination in compound preparation of traditional Chinese medicine.

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Objective To discuss the content determination method of α-asarone in Dianxianqing Granules. Methods Kromasil C18 column(250 mm×4.6 mm, 5 μm) was used, with methanol and water as mobile phase of gradient elution. The flow rate was 1.0 mL/min, and the detection wavelength was 257 nm. The column temperature was 25 ℃. Results The sample solution was prepared by reflux extracted with 70% ethanol for 20 min. α-asarone had a good linearity in the ranges of 1.24×10-3-7.44×10-3 μg(r =0.999 5). The method was quite good in precision, stability and repeatability, and the average recovery of α-asarone was 98.14%, with corresponding RSD of 2.5%. Conclusion The experiment provides an accurate and fast analytical method for α-asarone determination in compound preparation of traditional Chinese medicine.

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Available abstract

Objective To discuss the content determination method of α-asarone in Dianxianqing Granules. Methods Kromasil C18 column(250 mm×4.6 mm, 5 μm) was used, with methanol and water as mobile phase of gradient elution. The flow rate was 1.0 mL/min, and the detection wavelength was 257 nm. The column temperature was 25 ℃. Results The sample solution was prepared by reflux extracted with 70% ethanol for 20 min. α-asarone had a good linearity in the ranges of 1.24×10-3-7.44×10-3 μg(r =0.999 5). The method was quite good in precision, stability and repeatability, and the average recovery of α-asarone was 98.14%, with corresponding RSD of 2.5%. Conclusion The experiment provides an accurate and fast analytical method for α-asarone determination in compound preparation of traditional Chinese medicine.

Key concepts: Repeatability, Chromatography, Content determination, Elution, Chemistry, Gradient elution, Methanol, Ethanol content

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