Quality control of Kaixinsan based on drug-like components,3,6′-disinapoylsucrose and α-asarone

Objective To establish a method for quality control of Kaixinsan based on drug-like components,3,6′-disinapoylsucrose and α-asarone.Methods The procedure of HPLC coupled with photodiode array UV detector(HPLC-DAD) were performed on the chromatographic column of Venusil C18(4.6 mm×250 mm,5 μm),and the mobile phase was acetonitrile-0.1 % of phosphoric acid solution(42∶ 58) in gradient elution program.The flow velocity was 1 mL/min.The detection wavelength of 3,6′-disinapoylsucrose was 330 nm and that of α-asarone was 258 nm.The temperature was 30 ℃.Results 3,6′-Disinapoylsucrose showed a good linear relationship at range of 0.062 28 μg-0.373 68 μg(r=0.999 6) with average recovery as 98.35%(RSD=2.62%).α-Asarone showed a good linear relationship at range of 0.013 632 μg-0.136 320 μg(r=0.999 7) with average recovery as 96.65%(RSD=0.80%).Conclusion The quantitative determination of 3 batches of samples shows that the method is simple and accurate,which can be used in the quantitative determination of 3,6′-disinapoylsucrose and α-asarone and can be one of quality control methods for Kaixinsan.