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HPLC Analysis of Flavonoids in Flowers of Rosa Rugosa Thunb

Peng Yan-li

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Abstract

Objective:To establish the quantitative analysis method for determination of the contents of rutin,hyperoside and quercitrin in flowers of Rosa rugosa Thunb.Methods:HPLC analysis was performed on a Phenomenex Luna C18 column(250mm×4.6mm,5μm)with mobile phase acetonitrile-0.2% formic acid in gradient mode at a flow rate of 1mL/min,the detection wavelength was 360nm,and the column temperature was set at 35℃.Results:The linear ranges of rutin,hyperoside and quercitrin were the same as 0.04~1.0μg;their average recoveries were 97.5%(RSD=2.6%),96.3%(RSD=3.2%) and 97.1%(RSD=2.9%),respectively.Conclusion:The established method was simple,quick and reliable,it can be used to determine the contents of rutin,hyperoside and quercitrin in flowers of Rosa rugosa Thunb simultaneously,the results also provide a basis for the quality control of Rosa rugosa Thunb.

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Objective:To establish the quantitative analysis method for determination of the contents of rutin,hyperoside and quercitrin in flowers of Rosa rugosa Thunb.Methods:HPLC analysis was performed on a Phenomenex Luna C18 column(250mm×4.6mm,5μm)with mobile phase acetonitrile-0.2% formic acid in gradient mode at a flow rate of 1mL/min,the detection wavelength was 360nm,and the column temperature was set at 35℃.Results:The linear ranges of rutin,hyperoside and quercitrin were the same as 0.04~1.0μg;their average recoveries were 97.5%(RSD=2.6%),96.3%(RSD=3.2%) and 97.1%(RSD=2.9%),respectively.Conclusion:The established method was simple,quick and reliable,it can be used to determine the contents of rutin,hyperoside and quercitrin in flowers of Rosa rugosa Thunb simultaneously,the results also provide a basis for the quality control of Rosa rugosa Thunb.

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Available abstract

Objective:To establish the quantitative analysis method for determination of the contents of rutin,hyperoside and quercitrin in flowers of Rosa rugosa Thunb.Methods:HPLC analysis was performed on a Phenomenex Luna C18 column(250mm×4.6mm,5μm)with mobile phase acetonitrile-0.2% formic acid in gradient mode at a flow rate of 1mL/min,the detection wavelength was 360nm,and the column temperature was set at 35℃.Results:The linear ranges of rutin,hyperoside and quercitrin were the same as 0.04~1.0μg;their average recoveries were 97.5%(RSD=2.6%),96.3%(RSD=3.2%) and 97.1%(RSD=2.9%),respectively.Conclusion:The established method was simple,quick and reliable,it can be used to determine the contents of rutin,hyperoside and quercitrin in flowers of Rosa rugosa Thunb simultaneously,the results also provide a basis for the quality control of Rosa rugosa Thunb.

Key concepts: Hyperoside, Quercitrin, Rutin, Chromatography, Rugosa, Quercetin, High-performance liquid chromatography, Traditional medicine

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