Liquid Chromatographic Determination of Flavonoids in Rosa multiflora Thunb.from Xinjiang

A simple and rapid liquid chromatographic(LC) method was developed for determining total flavonoids in Rosa multiflora Thunb from Xinjiang.A mixture of methanol,isopropanol and deionized water was used for the chromatographic separation at a flow rate of 1 mL/min.The detection wavelength was set at 360 nm.Baseline separation was achieved for rutin and quercetin within 4 min.The contents of rutin and quercetin in Rosa multiflora Thunb from Xinjiang were 17.7 μg/g and 94.7μg/g(calculated on the basis of dry weight).Mean recoveries for rutin and quercetin at the spike level of 100μg/mL were 95.6% and 97.3%,respectively,and the RSDs were both below 5%.The repeatability of the developed method was good.As a conclusion,it is applicable for rapid determination of flavonoid compounds in Rosa multiflora Thunb,like rutin and quercetin.