Qualitative and quantitative analysis of the total flavonols in flowers of Rosa rugosa

Objective:To establish the qualitative and quantitative analysis method for the total flavonols in the flowers of Rosa rugosa.Methods:To detect quercetin and kaempferol by silica TLC with toluene - ethyl formateformic acid(10:8:1) as developing solvent and 3%aluminium trichloride alcoholic solution as visualization reagent; To determine the content of quercetin and kaempferol by HPLC,with C_(18) column Phenomenex Luna(250 mm×4.6 mm,5μm) and mobile phase methanol -0.4%phosphoric acid(60:40),at a flow rate of 1 mL·min~(-1) and detective wavelength 360 nm for assay.Results:Quercetin and kaempferol were detected by silica gel TLC.By HPLC,the linear range of quercetin was 0.06 - 0.6μg with a correlation coefficient 0.9996,the average recovery (n =5) was 99.2%with RSD of 3.9%;the linear range of kaempferol was 0.02 - 0.2μg with a correlation coefficient 0.9993,the average recovery(n =5) was 98.5%with RSD of 4.8%.Conclusion:Qualitative and quantitative analysis method for the total flavonols in the flowers of Rosa rugosa has been built up for the first time,the results also provide a basis for the quality control of Rosa rugosa.